Publication details

Pseudomonas leptonychotis sp. nov., isolated from Weddell seals in Antarctica

Authors

NOVÁKOVÁ Dana ŠVEC Pavel ZEMAN Michal BUSSE Hans-Jürgen MAŠLAŇOVÁ Ivana PANTŮČEK Roman KRÁLOVÁ Stanislava KRIŠTOFOVÁ Lucie SEDLÁČEK Ivo

Type Article in Periodical
Magazine / Source International Journal of systematic and Evolutionary Microbiology
MU Faculty or unit

Faculty of Science

Citation
Web https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003753
Doi http://dx.doi.org/10.1099/ijsem.0.003753
Keywords Pseudomonas leptonychotis; Antarctica; Weddell seal; polyphasic taxonomy
Attached files
Description A taxonomic study was carried out on four Gram-stain-negative strains P5773(T), P6169, P4708 and P6245, isolated from anus or mouth samples of Weddell seals at James Ross Island, Antarctica. The results of initial 16S rRNA gene sequence analysis showed that all four strains formed a group placed in the genus Pseudomonas and found Pseudomonas guineae and Pseudomonas peli to be their closest neighbours with 99.9 and 99.2% sequence similarity, respectively. Sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of isolates to P. peli (rpoD) and to P. guineae (rpoB and gyrB). The average nucleotide identity value below 86%, as calculated from the whole-genome sequence data, showed the low genomic relatedness of P5773(T) to its phylogenetic neighbours. The complete genome of strain P5773(T) was 4.4 Mb long and contained genes encoding proteins with biotechnological potential. The major fatty acids of the seal isolates were summed feature 8 (C-18:1 omega 7c), summed feature 3 (C-16 :1 omega 7c/C-16:1 omega 6c) and C-16:0. The major respiratory quinone was Q9. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Putrescine and spermidine are predominant in the polyamine pattern. Further characterization performed using repetitive sequence-based PCR fingerprinting and MALDI-TOF MS analysis showed that the studied isolates formed a coherent cluster separated from the remaining Pseudomonas species and confirmed that they represent a novel species within the genus Pseudomonas, for which the name Pseudomonas leptonychotis sp. nov. is suggested. The type strain is P5773(T) (=CCM 8849(T)=LMG 30618(T)).
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