Publication details

Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes

Authors

MYLENKO Mykola VU Dai Long KUTA Jan RANGLOVA Karolina KUBÁČ David LAKATOS Gergely GRIVALSKY Tomas CAPORGNO Martin Pablo MANOEL Joao Artur Camara KOPECKY Jiri MASOJÍDEK Jiří HROUZEK Pavel

Year of publication 2020
Type Article in Periodical
Magazine / Source Journal of Agricultural and Food Chemistry
MU Faculty or unit

Faculty of Science

Citation
Web https://pubs.acs.org/doi/10.1021/acs.jafc.9b06196
Doi http://dx.doi.org/10.1021/acs.jafc.9b06196
Keywords Chlorella; microalgae; selenium; selenomethionine; selenocysteine; methylselenocysteine; phototrophic cultivation; heterotrophic cultivation
Description Microalgae accumulate bioavailable selenium-containing amino acids (Se-AAs), and these are useful as a food supplement. While this accumulation has been studied in phototrophic algal cultures, little data exists for heterotrophic cultures. We have determined the Se-AAs content, selenium/sulfur (Se/S) substitution rates, and overall Se accumulation balance in photo-and heterotrophic Chlorella cultures. Laboratory trials revealed that heterotrophic cultures tolerate Se doses, similar to 8-fold higher compared to phototrophic cultures, resulting in a similar to 2-3-fold higher Se-AAs content. In large-scale experiments, both cultivation regimes provided comparable Se-AAs content. Outdoor phototrophic cultures accumulated up to 400 mu g g(-1) of total Se-AAs and exhibited a high level of Se/S substitution (5-10%) with 30-60% organic/total Se embedded in the biomass. A slightly higher content of Se-AAs and ratio of Se/S substitution was obtained for a heterotrophic culture in pilot-scale fermentors. The data presented here shows that heterotrophic Chlorella cultures provide an alternative for Se-enriched biomass production and provides information on Se-AAs content and speciation in different cultivation regimes.
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