Publication details

Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis

Authors

BOŘILOVÁ LINHARTOVÁ Petra DANĚK Zdeněk DEISSOVÁ Tereza HROMČÍK Filip LIPOVÝ Břetislav SZÁRAZ Dávid JÁNOŠ Július FASSMANN Antonín BARTOVA Jirina DRIZHAL Ivo IZAKOVIČOVÁ HOLLÁ Lydie

Year of publication 2020
Type Article in Periodical
Magazine / Source International Journal of Molecular Sciences
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.mdpi.com/1422-0067/21/13/4728
Doi http://dx.doi.org/10.3390/ijms21134728
Keywords aggressive periodontitis; oral bacteria; inflammation; interleukin; polymorphism; genetic predisposition
Description Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (IL) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms inIL-1gene cluster,IL-6and its receptor,IL-10,IL-17A, andIL-18were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphismIL-10-1087 A/G (rs1800896) and specificIL-10haplotypes were associated with the development of the disease (p< 0.05,P-corr> 0.05). Four bacterial species occurred more frequently in AgP than in controls (p< 0.01,P-corr< 0.05). Elevated IL-10 levels were found in AgP patients, carriers ofIL-10-1087GG genotype, and PBMCs stimulated by periodontal bacteria (p< 0.05,P-corr> 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression ofIL-10and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development.
Related projects: