Publication details

The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis

Authors	SMITH S. ZHU S.S. JOOS L. ROBERTS I. NIKONOROVA N. VU L.D. STES E. CHO H. LARRIEU A. XUAN W. GOODALL B. VAN DE COTTE B. WAITE J.M. RIGAL A. HARBOROUGH S.R. PERSIAU G. VANNESTE S. KIRSCHNER G.K. VANDERMARLIERE E. MARTENS L. STAHL Y. AUDENAERT D. FRIML Jiří FELIX G. SIMON R. BENNETT M.J. BISHOPP A. DE JAEGER G. LJUNG K. KEPINSKI S. ROBERT S. NEMHAUSER J. HWANG I. GEVAERT K. BEECKMAN T. DE SMET I.
Year of publication	2020
Type	Article in Periodical
Magazine / Source	MOLECULAR & CELLULAR PROTEOMICS
MU Faculty or unit	Central European Institute of Technology
Citation
Web	https://www.sciencedirect.com/science/article/pii/S1535947620349604?via%3Dihub
Doi	http://dx.doi.org/10.1074/mcp.RA119.001826
Keywords	ENHANCES DROUGHT TOLERANCE; AUXIN RESPONSE; NUCLEAR-LOCALIZATION; OSMOTIC-STRESS; REGULATE ROOT; RECEPTOR; WATER; GENE; EXPRESSION; KINASES
Description	Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabi-lizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.