Publication details

Cdk11 is a Novel Player in Non-coding RNA Expression

Authors

HLUCHÝ Milan POTĚŠIL David ZDRÁHAL Zbyněk BLAŽEK Dalibor

Year of publication 2020
Type Conference abstract
MU Faculty or unit

Central European Institute of Technology

Citation
Description Cdk11 is a poorly-characterized cyclin-dependent kinase with described functions in transcription of mRNA, alternative splicing and 3’end processing of HIV mRNA. Our aim was to better define its cellular roles via identification of novel interacting partners and candidate substrates combined with data from microarray experiment after knock-down of Cdk11. We utilized BioID method to probe for relevant proteins connected to Cdk11. Microarray data showed differential expression of a subset of non-coding genes. Proteins implicated in control of their expression and processing were identified in BioID screen as candidate interacting partners of Cdk11. We utilized also Cdk11 analog-sensitive mutant (designed using Shokat’s lab approach) to test the candidate interacting partners as substrates of Cdk11 in vitro. Altogether, our data uncover novel role of Cdk11 in the expression of subset of non-coding RNAs
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