Publication details

Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography

Authors

PAZOUREK Jiří

Year of publication 2014
Type Article in Periodical
Magazine / Source Carbohydrate Research
MU Faculty or unit

Faculty of Pharmacy

Citation
Web https://www.sciencedirect.com/science/article/abs/pii/S0008621514001050
Doi http://dx.doi.org/10.1016/j.carres.2014.03.010
Keywords myo-inositol; HILIC; ELSD
Description A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, D-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo-and D-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12 min at 10 degrees C, though higher temperatures (25 degrees C or 40 degrees C) or higher water content in the mobile phase could speed up the separation and determination to four minutes. LOD of the method was 9 mg/L at 10 degrees C, and 5 mg/L at 25 degrees C, resp. (C) 2014 Elsevier Ltd. All rights reserved.

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