Publication details

Osmolarita slz, její měření a korelace s dalšími testy pro vyšetření suchého oka

Title in English Tear osmolarity, its measurement and correlation with other tests for dry eye examination


Year of publication 2021
MU Faculty or unit

Faculty of Medicine

Description Abstract Aim: To determine the values of osmolarity of tears of the right and left eye in individual probands, to evaluate their symptoms using the DEQ-5 questionnaire and to compare the results with other studies. Furthermore, to find out how invasive and non-invasive methods of tear film quality evaluation correlate together. Methods: In our study, we had data from 20 probands, whose average age was 23.5 years (SD 1.55 years). All subjects underwent a standard optometric examination, including the determination of the current subjective correction of the refractive error. Subsequently, we presented the DEQ-5 questionnaire to the probands. After completing the questionnaires, each proband underwent tear osmolarity using a TearLab® instrument (TearLab Corporation, USA). Further examination was performed on a Keratograph 5M® (Oculus, Germany). Results: The average value of tear osmolarity in the right and left eye of all probands was 300.35 (SD 16.52) and 293.70 (SD 12.92) mOsmol/l, respectively. The difference in the osmolarity of both eyes in one proband greater than 8 mOsmol/l inclusive was identified in a total of 11 probands out of 20 (i.e. 55 %). The mean value of the non-invasive break time test (nBUT) on the right and left eyes of all probands was 14.05 s (SD 5.78 s) and 15.59 s (SD 6.13 s), respectively. From the DEQ-5 questionnaire, we recorded a positive capture (score equal to greater than 6 points) in a total of 8 probands out of 20 (i.e. 40 %). Conclusion: The results of our study show that there is no correlation between non-invasive break time test (nBUT) and tear osmolarity (difTL8, R = 0.23 at p = 0.32). To evaluate the symptoms of dry eye disease, a special questionnaire with 5 questions (Dry Eye Questionnaire, DEQ-5) proved to be very accurate and effective, which helped us to accurately divide the baseline group into a symptomatic group and a group without symptoms (p <0.001). Subsequently, we demonstrated statistically significant sensitivity and specificity in both tests (nBUT, difTL8).
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