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PMVEMA-coated upconverting nanoparticles for upconversion-linked immunoassay of cardiac troponin

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SHAPOVAL Oleksandr BRANDMEIER Julian NAHORNIAK Mykhailo OLEKSA Viktoriia MAKHNEVA Ekaterina GORRIS Hans-Heiner FARKA Zdeněk HORÁK Daniel

Year of publication 2022
Type Article in Periodical
Magazine / Source Talanta
MU Faculty or unit

Faculty of Science

Keywords Photon-upconversion nanoparticle; Poly(methyl vinyl ether-alt-maleic acid);Bioconjugation; Immunoassay; Upconversion-linked immunosorbent assay; Cardiac troponin I
Description Surface engineering of upconverting nanoparticles (UCNPs) is crucial for their bioanalytical applications. Here, an antibody specific to cardiac troponin I (cTnI), an important biomarker for acute myocardial infection, was covalently immobilized on the surface of UCNPs to prepare a label for the detection of cTnI biomarker in an upconversion-linked immunoassay (ULISA). Core-shell UCNPs (NaYF4:Yb,Tm@NaYF4) were first coated with poly(methyl vinyl ether-alt-maleic acid) (PMVEMA) and then conjugated to antibodies. The morphology (size and uniformity), hydrodynamic diameter, chemical composition, and amount of coating on the of UCNPs, as well as their upconversion luminescence, colloidal stability, and leaching of Y3+ ions into the surrounding media, were determined. The developed ULISA allowed reaching a limit of detection (LOD) of 0.13 ng/ml and 0.25 ng/ml of cTnI in plasma and serum, respectively, which represents 12- and 2-fold improvement to conventional enzyme-linked immunosorbent based on the same immunoreagents.
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