Publication details

Microfluidic device integrating single-cell extraction and electrical lysis for mass spectrometry detection of intracellular compounds

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Authors

VÁCLAVEK Tomáš FORET František

Year of publication 2022
Type Article in Periodical
Magazine / Source Electrophoresis
MU Faculty or unit

Faculty of Science

Citation
Web https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/full/10.1002/elps.202100379
Doi http://dx.doi.org/10.1002/elps.202100379
Keywords bioanalysis; hemoglobin; mass spectrometry; microfluidics; single-cell analysis
Description Analysis of cellular composition and metabolism at a single-cell resolution allows gaining more information about complex relationships of cells within tissues or whole living organisms by resolving the variance stemming from the cellular heterogeneity. Mass spectrometry (MS) is a perfect analytical tool satisfying the demanding requirements of detecting and identifying compounds present in such ultralow-volume samples of high chemical complexity. However, the method of sampling and sample ionization is crucial in obtaining relevant information. In this work, we present a microfluidic sampling platform that integrates single-cell extraction from MS-incompatible media with electrical cell lysis and nanoESI-MS analysis of human erythrocytes. Hemoglobin alpha and beta chains (300 amol/cell) were successfully identified in mass spectra of single-erythrocyte lysates.
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