Publication details

Individual copy number variation and extensive diversity between major MHC-DAB1 allelic lineages in the European bitterling



Year of publication 2022
Type Article in Periodical
Magazine / Source Immunogenetics
MU Faculty or unit

Faculty of Science

Keywords Rhodeus amarus; MHC class IIB; Positive selection; Gene duplication; MHC supertypes; Positively selected sites
Description Polymorphism of the major histocompatibility complex (MHC), DAB1 gene was characterized for the first time in the European bitterling (Rhodeus amarus), a freshwater fish employed in studies of host-parasite coevolution and mate choice, taking advantage of newly designed primers coupled with high-throughput amplicon sequencing. Across 221 genotyped individuals, we detected 1-4 variants per fish, with 28% individuals possessing 3-4 variants. We identified 36 DAB1 variants, and they showed high sequence diversity mostly located within predicted antigen-binding sites, and both global and codon-specific excess of non-synonymous mutations. Despite deep divergence between two major allelic lineages, functional diversity was surprisingly low (3 supertypes). Overall, these findings suggest the role of positive and balancing selection in promotion and long-time maintenance of DAB1 polymorphism. Further investigations will clarify the role of pathogen-mediated selection to drive the evolution of DAB1 variation.

You are running an old browser version. We recommend updating your browser to its latest version.

More info