Publication details

Influence of protein corona on the interaction of glycogen-siRNA constructs with ex vivo human blood immune cells

Authors

WOJNILOWICZ Marcin LÁZNIČKOVÁ Petra JU Yi ANG Ching-Seng TIDU Federico BENDICKOVA Kamila FORTE Giancarlo PLEBANSKI Magdalena CARUSO Frank CAVALIERI Francesca FRIČ Jan

Year of publication 2022
Type Article in Periodical
Magazine / Source BIOMATERIALS ADVANCES
MU Faculty or unit

Faculty of Medicine

Citation
Web https://www.sciencedirect.com/science/article/pii/S2772950822003600?via%3Dihub
Doi http://dx.doi.org/10.1016/j.bioadv.2022.213083
Keywords Glycogen nanoparticles; siRNA glycoplexes; Peripheral blood mononuclear cells; THP-1; Phagocytosis; Protein corona; Stochastic optical reconstruction microscopy
Description Glycogen-nucleic acid constructs i.e., glycoplexes are emerging promising platforms for the alteration of gene expression and transcription. Understanding the interaction of glycoplexes with human blood components, such as serum proteins and peripheral blood mononuclear cells (PBMCs), is important to overcome immune cell activation and control biodistribution upon administration of the glycoplexes in vivo. Herein, we investigated the interactions of polyethylene glycol (PEG)ylated and non-PEGylated glycoplexes carrying siRNA molecules with PBMCs isolated from the blood of healthy donors. We found that both types of glycoplexes were non-toxic and were primarily phagocytosed by monocytes without triggering a pro-inflammatory interleukin 6 cytokine pro-duction. Furthermore, we investigated the role of the protein corona on controlling the internalization efficiency in immune cells - we found that the adsorption of serum proteins, in particular haptoglobin, alpha-1-antitrypsin and apolipoprotein A-II, onto the non-PEGylated glycoplexes, significantly reduced the uptake of the glycoplexes by PBMCs. Moreover, the non-PEGylated glycoplexes were efficient in the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) knockdown in monocytic THP-1 cell line. This study provides an insight into the rational design of glycogen-based nanocarriers for the safe delivery of siRNA without eliciting unwanted immune cell activation and efficient siRNA activity upon its delivery.

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