Publication details
Využití PureMilk testů při hodnocení mikrobiologické kvality mléka z mléčných automatů
| Title in English | The use of PureMilk tests in the evaluation of the microbiological quality of milk from milk machines |
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| Authors | |
| Year of publication | 2022 |
| Type | Article in Periodical |
| Magazine / Source | Veterinářství |
| Citation | |
| Description | The aim of the study was to evaluate the microbiological quality and safety of raw cow's milk sold through milk vending machines in Brno and Kuřim. Samples were taken from five vending machines in Brno and Kuřim supplied by two farms. A total of 210 raw cow's milk samples were taken between August 2017 and September 2021. Staphylococcus aureus, Escherichia coli and Enterococcus faecalis were determined in the samples using the PureMilk test. The presence of sea–sej genes encoding enterotoxin production was detected in S. aureus bacteria and the presence of stx1 and stx2 genes encoding shigatoxin production was detected in E. coli bacteria by multiplex PCR (polymerase chain reaction). S. aureus was detected in 41 samples (19.5%). Legislative limit for S. aureus (500 CFU/ml) required by Decree No. 289/2007 Coll. as amended, was exceeded in three samples (7.3%). The presence of genes for the production of staphylococcal enterotoxins (seb, seg and sei) was demonstrated in 12 samples (29.3%). Ofthe 210 samples, the presence of E. coli was detected in 61 samples (29.0%). The number of E. coli in taken samples ranged from 0 to 3.70 log CFU/ml. Enterococcus faecalis was determined in 140 samples (66.7%). Its number ranged from 0 to 3.48 log CFU/ml. The obtained values were statistically compared between individual farms and milk vending machines. No statistically significant differences were found in any of the monitored bacteria in relation to the numbers in summer and winter. |