Publication details

Functional tests of B cells are independent of EUROclass classification in CVID patients

Authors

ŠTÍCHOVÁ Julie SLANINA Peter VLKOVÁ Marcela

Year of publication 2019
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Citation
Description METHODOLOGY: B cells from 31 CVID patients and 21 healthy donors were stimulated through T dependent and T independent signaling pathway in vitro. The ability of differentiation, proliferation, expression of inhibitory Fc?IIB receptor (CD32B), IL-10 receptor (CD210) and IL-10 production was measured by eight color flow cytometry after a seven-day incubation. The production of isotype switched immunoglobulins was determined by ELISA method. RESULTS: We observed an increased frequency of naive B cells and decreased frequency of memory B cells and plasmablasts in CVID patients when compared to healthy donors. Proliferation was reduced in CD21low B cells and plasmablasts, whereas naive and memory B cells showed normal proliferative activity. The expression of inhibitory Fc?IIB receptor in CVID patients was comparable to healthy donors among all B cell subsets with an exception of increased CD32B expresion on CD21low B cells. It is plausible that low concentrations of IgG in CVID patients may lead to the restriction of CD32B inhibitory effect in CD21low B cell development. In one CVID patient, we revealed significantly higher expression of CD32B on marginal zone B cells and plasmablasts after T dependent stimulation. This patient is undergoing detailed research of CD32B gene. The expression of IL-10 receptor was slightly increased in plasmablasts, marginal zone B cells and CD21low B cells. Production of IL-10 was decreased among all B cell subsets after T independent stimulation, but after T dependent stimulation only plasmablasts produced less IL-10. CONCLUSION: A diverse ability of B cell differentiation into plasmablast stage was observed in CVID patients. This ability was independent of the EUROclass classification.

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