Pseudomonas aeruginosa bacteriophages isolated from hospital wastewater and their effect on healthcare-associated strains
|Year of publication
|MU Faculty or unit
|The drug-resistant strains Pseudomonas aeruginosa are the cause of serious and even life-threatening infections. In this case, the application of bacteriophages is hopeful alteration of antibiotic therapy. New phages for therapy could be obtained from environment, when the one of the suitable sources for phage isolation are wastewaters. In this study, we have focused on isolation and characterisation of new bacteriophages with therapeutic potential from wastewater that has same origin (i. e. hospital) as phage host strains of P. aeruginosa. Firstly, we isolated five new pseudomonal bacteriophages from samples of wastewater acquired from UHO (University Hospital Ostrava). We determined the lytic patterns of these phages against 26 UHO isolates of P. aeruginosa together with nineteen pseudomonal phages from FAGOFARMA company collection. Overall the 80.8 % of strains were sensitive (i. e. at least one of the assessed phages formed plaques with these strains). After that, we applied sequencing, PCR, and transmission electron microscopy for phage taxonomic classification. The phages from hospital wastewaters were characterised as members of Pbunavirus and Nankokuvirus genera. According to literature, similar phages are commonly use in commercial therapeutic cocktails. In conclusion, we proved the possible isolation of bacteriophages with therapeutic potential from Czech hospital wastewaters. In the future, these phages can be components of cocktail for treatment of infections caused by drug-resistant strains of P. aeruginosa.