Publication details
Use of all-male triploid population of zebrafish (Danio rerio) for evaluation of 17 alpha-ethinylestradiol effects
| Authors | |
|---|---|
| Year of publication | 2022 |
| Type | Article in Periodical |
| Magazine / Source | Neuroendocrinology letters |
| MU Faculty or unit | |
| Citation | |
| Web | https://www.nel.edu/userfiles/articlesnew/1674499217_43_5_weiserova_281-pdf.pdf |
| Keywords | EE2; feed contamination; oral intake; vitellogenin; xenoestrogen testing |
| Description | OBJECTIVES: We tested the toxicity of ethinylestradiol, a semisynthetic estrogen used in oral contraceptives, on all-male triploid zebrafish using commercial feeds and three different doses concentrations. We aimed to determine whether ethinylestradiol peroral administration resulted in vitellogenin production and whether all-male triploid zebrafish could serve as a model species for xenoestrogen testing. METHODS: The actual concentrations of 17 alpha-ethinylestradiol were 0.0035 (low); 0.0315 (medium) and 0.365 (high) mu g/g. Positive control represented commercial feeds containing 0.0465 mu g/g of beta-estradiol. The experiment lasted 8 weeks. RESULTS: Our results indicate that 17 alpha-ethinylestradiol consumption does induce vitellogenin production in triploid zebrafish. CONCLUSIONS: The simple presence of vitellogenin is a definite symptom indicative of the potential for such changes due to the action of estrogenic substances. As such, this experiment has shown that the use of all-male triploid zebrafish populations, rather than the mixed-sex populations of other species previously used, could serve as a suitable alternative model population for controlled testing of the effects of xenoestrogens on fish. |