Publication details
Polymerázová řetězová reakce v mikrobiologické diagnostice
| Title in English | Polymerase Chain Reaction in Microbiology |
|---|---|
| Authors | |
| Year of publication | 2000 |
| Type | Article in Periodical |
| Magazine / Source | Praktický lékař |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | polymerase chain reaction - microbiological diagnostics |
| Description | In microbiology, the PCR is employed above all for the detection of microbial DNA in the examined specimen. The greatest advantage of PCR is the speed and the ability to detect even the non-cultivable microbes. Therefore, it is used in situations, where classical microbiological techniques fail. PCR is also very sensitive and relatively specific. However, it is necessary to consider also possible false positivity and false negativity. In comparison with classical techniques the PCR is relatively expensive. The paper gives examples of practical application of PCR. |