Publication details
Využití imunomagnetické separace při detekci bakterií rodu Salmonella pomocí polymerázové řetězové reakce.
| Title in English | The use of immunomagnetic separation for detection of bacteria of genus Salmonella by polymerase chain reaction. |
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| Authors | |
| Year of publication | 2002 |
| Type | Article in Proceedings |
| Conference | VI. pracovní setkání biochemiků a molekulárních biologů |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | Salmonella cells; milk; IMS; IMS-PCR |
| Description | The aim of work was to elaborate saving procedure enabling IMS and IMS-PCR estimation of Salmonella cells from artificially contaminated milk. Supermagnetic polystyrene particles Dynabeads anti-Salmonella were used for isolation of Salmonella cells. The efficiency of IMS was estimated after plating of captured cells on LB agar plates and using PCR. Procedure of IMS and IMS-PCR of Salmonella cells was optimalised with respect to time and material used. |