Publication details
Štúdium nukleových kyselín v podchladenej vode pomocou NMR metód.
| Title in English | NMR Study of Nucleic Acids In Supercooled Water |
|---|---|
| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | Sborník abstraktů, Studentská vědecká konference v oboru chemie a biochemie |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | NMR;DNA;structure |
| Description | Short DNA hairpins play a significant role in a number of biological processes. The most interesting feature of the oligonucleotides with a general sequence d(GCGNAGC) (N=A,G,C,T) is their extraordinary stability represented by high melting temperatures, polyacrylamide gel mobility, and resistance against nucleases. Detailed knowledge of these structures helps understand their unique behavior. One of these fragments, the d(GCGAAGC) structure has been solved previously by NMR spectroscopy using the NOE-derived distances, torsion angles, and residual dipolar couplings. The aim of the present study is a new structure calculation of d(GCGAAGC) based on NOE data measured at temperatures below 273 K when the intramolecular motions and the chemical exchange of the amino protons are inhibited. We focused here on a comparison of number of restraints derived from the NOESY spectra at 268K and 303K, paying our attention to amino and imino connectivities within a molecule. |
| Related projects: |