Ribotyping of sucrose - negative mesophilic Aeromonas sp.
|Year of publication||2005|
|MU Faculty or unit|
|Description||Aeromonads are ubiquitous waterborne organisms isolated from both environmental sources and clinical material. During the past few years the taxonomy of the genus Aeromonas has been changed and several new species were proposed. Methods used to type aeromonads are based either on phenotypic characteristics or on the analysis of their genomes. In total 11 sucrose-negative isolates of aeromonads were obtained from fresh water in 2004. Biochemical identification by commercial kit ENTEROtest24 (Pliva-Lachema) was supplemented with additional tube and plate tests and results were evaluated by software TNW (Pliva-Lachema). Ribotyping of selected isolates and reference cultures using EcoRI and HindIII restriction enzymes and DNA probe complementary to 16S and 23S rRNA was accomplished. Majority species of the genus Aeromonas produce acid from sucrose except A. jandaei, A. schubertii, A. trota or A. eucrenophila species. Biochemical identification to the species level of isolates with negative acid production from sucrose was unsuccessful. Biotyping confirmed that all 11 isolates belong to the genus Aeromonas. Ribotyping profiles from EcoRI and HindIII of analysed isolates gave unique band pattern with no relationship to the selected reference aeromonas cultures. Five sucrose-negative isolates with positive acid production from inositol demonstrated good correlation among results of phenotyping and ribotyping in this study and differed from the remaining six isolates which shoved heterogeneous hybridization profiles. Ribotyping appeared to be a good tool for the differentiation of aeromonads and mentioned five isolates with unique biotype probably represent a novel species.|