Publication details
Determination of Trinitrotoluenes and product of their Biotransformation using liquid chromatography and Capillary Electrophoresis
| Authors | |
|---|---|
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Chemické Listy |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | Chromatogrphy; Electrophoresis; Trinitrotoluene |
| Description | Contamination by the explosive as 2,4,6-trinitrotoluene (TNT), is often associated with former and present munitions manufacturing facilities, storage depots, and former sites of explosives use.1 TNT is widely distributed in enviroment and there can be persistent for very long times or slowly degradation in the water and in the soil, influenced by light, microorganisms, oxygen and reducing chemicals. TNT and their products of degradation, aminodinitrotoluenes (ADNT), diaminonitrotoluenes (DANT) and triaminotoluene (TAT) have been found to be cytotoxic presumably due to induced oxidative stress and demonstrate mutagenic capability (EPA classifies TNT as possible human carcinogens).2,3 Complete separation of these structurally similar compounds is necessary in order to their accurately determine and quantify in environmental samples and in (bio)transformation studies. High performance liquid chromatography (HPLC) has remained the major analytical tool for the detection and quantification of nitroaromatic compounds.4 At present, capillary electromigration methods, capillary electrochromatography (CEC) and micellar electrokinetic chromatography (MEKC) enable to separate these compound with good separation efficiency. |
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