The identification of Gyrodactylus species parasitising Barbatula barbatula L., 1758 in Central Europe: combination morphologic and molecular methods.
|Year of publication||2005|
|Type||Article in Proceedings|
|Conference||5th International Symposium on Monogenea|
|MU Faculty or unit|
|Keywords||Gyrodactylus; Barbatula barbatula|
|Description||During 2001, Gyrodactylus specimens were collected from Barbatula barbatula originated in Haná River and two sampling sites on Vlára River, Czech Republic, in monthly samples of fifteen fishes. Thirty-one specimens of Gyrodactylus were from different seasons were stored for molecular analysis. Sequencing of the complete internal transcribed spacer (ITS) rRNA genes of Gyrodactylus specimens parasitising B. barbatula from locality Haná revealed sequence differences on the species level. According to the sequence comparison, the presence of at least three Gyrodactylus jiroveci-like species was predicted. Further analysis was required to confirm existence these species according to standard morpholometrical description, using drawings and measurements of the hard part of parasite haptor. According to the shape of marginal hook sickle, the specimens were divided into 3 groups. Measurements of the hard parts of haptor (ten characters) from two species-groups (80 specimens from four seasons) were analysed. Using K mean cluster test and PCA plot, two species-groups were discriminated into two isolated group. Results of this preliminary analysis suggest presence of G. jiroveci and Gyrodactylus papernai (recently used as synonymum for G. jiroveci) in central europeans rivers. The third species-group was determined as Gyrodactylus pseudonemachili that is originally described from Asia, and this species seemed to occurred very rarely in Central Europe. Due to limited number of collected specimens of G. pseudonemachili, this species was not included in metrical analyses.|