Publication details
Enkapsulace tří druhů entomopatogenních hlístovek u G. mellonella a larev brouků čeledi Scarabaeidae
| Title in English | Encapsulation of three species of entomopathogenic nematodes in G. mellonella and Scarabaeid grubs |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Zborník zo VII. vedeckej konferencie doktorandov a mladých vedeckých pracovníkov |
| MU Faculty or unit | |
| Citation | |
| Description | Encapsulation is one from the most effective cellular defence mechanisms in insects against entomopathogenic nematodes (EPNs). The pathogenesis of Heterorhabditis bacteriophora, Steinernema scarabaei and Steinernema glaseri against Galleria mellonella and scarabaeids (Amphimallon sp. rsp. Hoplia sp.) was compared. Scarabaeidae are very resistant to EPNs except Steinernema scarabaei, recently described specie from the USA. Interestingly this nematode has a low effectivity against G. mellonella, an insect which is highly susceptible to most known EPNs. Encapsulation was detected microscopically after natural invasion as well as after injection of EPNs. Positive results were obtained in G. mellonella (infected by H. bacteriophora) and Amphimallon sp. (after injection of H. bacteriophora or S. scarabaei). No encapsulation was observed in Hoplia sp. The encapsulation response varied considerably between individual insects. On the average, app. 10 % of EPNs were encapsulated. Encapsulation (cellular without melanization) was mainly initiated at the tail region of the EPN, detected capsules were disappearing. In all cases, encapsulation was not apparent in S. glaseri - infected insect. To sumarize, relationship between pathogenity and encapsulation of particular species of EPNs in host insect was demonstrated. This work was done in cooperation with Institute of Phytopathology, Department of Biotechnology and Biological Control, University Kiel, Germany. |