Publication details
Avidin a streptavidin - možnosti jejich elektroanalytického stanovení
| Title in English | Avidin and streptavidin - ways how they can be determined using electroanalytically techniques |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Vyziva zvirat 2006 - PROTEINY |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Avidin; Biotin; streptavidin; electrochemical detection; bacteria Streptomyces avidinii; ELISA |
| Description | Avidin, a minor constituent of egg white from reptiles, amphibians and birds, is a glycosylated and positively charged protein (at neutral pH), which usually forms tetramers. Few years later after discovering of avidin, the protein, whose sequence is evolutionary related to that of avidin, was isolated from bacteria Streptomyces avidinii. Avidin/streptavidin combines selectively with biotin. This interaction has been utilizing in many types of avidin-biotin technologies, such as imunohistochemistry, electron microscopy, ELISA, DNA hybridization and construction of biosensors. Suggesting of advantageous methods for the analysis of the avidin/streptavidin-biotin interaction is need. In this paper we describe the preparation of avidin- and avidin extract-modified CPEs for sensitive avidin detection in solution and in transgenic maize plants. We also describe the detection of streptavidin by means of the electrochemical method. |
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