Publication details
Vyšetření sér myší BALB/c na přítomnost antiborreliových protilátek metodami ELISA a immunoblotting po imunizaci různými kmeny patogenní Borrelia afzelii
| Title in English | Examination of BALB{c mice sera on the presence of antiborrelian antibodies using ELISA nad immunoblotting methods after immunization with various strains of pathogenic Borrelia afzelii |
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| Authors | |
| Year of publication | 2006 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Immunization of BALB/c mice with three different stems of Borrelia afzelii obtained from Ixodes ricinus tick, Culef (Culex) pipiens molestus mosquitoe and Apodemus flavicollis mouse. Detection of antibodies using ELISA method and proteins responsible for the production of antibodies by imunoblotting. The level of produced antibodies was similar in all 3 groups. The immunogenic proteins varied among groups (stems). The most significant reaction was found against OspA and OspC. Production of IgM antibodies against OspC prevailed in the early phase. OspA induced production of IgG in the late phase of immunization. |
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