Publication details
Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Proteomics |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | Bovine trypsin; Conjugate; In-gel digestion; Oligosaccharide; PMF |
| Description | Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins. |
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