Publication details
Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9 |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | Magnetic particles; HEMA-co-GMA; Bifidobacterium; RAPD; rep-PCR; reproducibility |
| Description | DNA was isolated from Bifidobacterium cells using phenol extraction metod, salting out using 6 M NaCl, and adsorption on magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (HEMA-co-GMA) particles containing carboxyl groups. It was shown that DNA eluted from magnetic particles into TE buffer can be succesfully used in molecular fingerprinting procedures. The reproducibility of tested methods were comparable with phenol extraction method. |
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