Use of magnetic hydrophilic particles for DNA isolation from dairy products and unusual samples

• Authors: ŠPANOVÁ Alena; RITTICH Bohuslav; HORÁK Daniel; RYBNIKÁŘ Alois; ŠTYRIAK Igor; JŮZOVÁ V.; ROHÁNKOVÁ L.; RUTTKAY-NEDECKÝ B.; KUČERA P.
• Year of publication: 2006
• Type: Article in Proceedings
• Conference: Ninth International symposium on hyphenated techniques in Chromatography and Hyphenated Chromatographic analyzers, HTC-9
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: Magnetic particles; DNA; dairy products; Trychophyton cells; Lake Baikal sediments; GMO; PCR
• Description: Magnetic hydrophilic methacrylate–based polymer microspheres containing carboxyl groups were used for isolation of bacterial DNA from differnt samples. DNA was adsorbed on particles in the presence of 8 wt. % of poly(ethylene glycol) (PEG 6,000) and 2 M NaCl concentrations. The eluted DNA was used directly for PCR identification of Salmonella cells containing intracellular PCR inhibitors, Lactobacillus and Bifidobacterium cells in dairy products (cheese, fermented dairy products), Trichophyton cells in lyophilisates, bacterial cells in sediments from Lake Baikal, and GMO testing (DNA isolated from rose soya granulate).

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