Publication details
Detekce neobvyklé mutace nádorového supresoru p53 u Burkittova lymfomu: duplikace 30 pb
| Title in English | An unusual p53 mutation detected in Burkitts lymphoma: 30 bp duplication |
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| Authors | |
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | XIV. Jihočeské onkologické dny |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | Burkittův lymfom; p53; FASAY; duplikace |
| Description | Burkitts lymphomas are aggressive fast growing tumors. Alterations of the p53 tumor suppressor occur frequently in this disease. By immunohistochemical analyses, we detected high level of the p53 protein in the tumor tissue. Immunoblotting analysis showed higher molecular weight of the p53 protein overexpressed in tumor tissues than that of the standard p53 protein. Functional analyses of separated alleles in yeast clearly revealed that the tumor variant of the p53 protein was transcriptionally nonfunctional. The yeast colonies expressing this p53 variant possessed unique phenotype. Sequencing of the tumor cDNA revealed duplication of the 30 bp region of the p53 gene. |
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