Characterization of Haloalkane Dehalogenases DrbA and DmbC: Representatives of Novel Subfamily.

• Title in English: Biochemical Characterization of Haloalkane Dehalogenases DrbA and DmbC: Representatives of Novel Subfamily.
• Authors: JESENSKÁ Andrea; MONINCOVÁ Marta; KOUDELÁKOVÁ Táňa; HASAN Khomaini; CHALOUPKOVÁ Radka; PROKOP Zbyněk; GEERLOF Z.; DAMBORSKÝ Jiří
• Year of publication: 2009
• Type: Article in Periodical
• Magazine / Source: Applied and Environmental Microbilogy
• MU Faculty or unit: Faculty of Science
• Web: http://loschmidt.chemi.muni.cz/peg/
• Field: Biochemistry
• Keywords: Isolation; Biochemical Characterization; Haloalkane Dehalogenases; DrbA and DmbC; Subfamily.
• Description: This study focuses on two representatives of experimentally uncharacterized haloalkane dehalogenases from the subfamily HLD-III. We report biochemical characterization of the expression products of haloalkane dehalogenases genes drbA from Rhodopirellula baltica SH1 and dmbC from Mycobacterium bovis 5033/66. The DrbA and DmbC enzymes show highly oligomeric structure and very low activity with typical substrates of haloalkane dehalogenases.

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