Publication details

Changes of level of endogenous phytohormones as new marker of environmental loading



Year of publication 2007
Type Article in Proceedings
Conference MendelNet 2007 Agro - Sborník z mezinárodní konference posluchačů postgraduálního doktorského studia
MU Faculty or unit

Faculty of Science

Field Physiology
Keywords fluoranthene; phytohormone; pea plants; in vitro;
Description The aim of our study was to find, establish and verify new methods for early indication of stress evoked by organic compounds in plants. The level of endogenous phytohormones in pea plants (Pisum sativum L. cv. Garde) cultivated in vitro in the presence of polycyclic aromatic hydrocarbon (PAH) fluoranthene (FLT; 0.1, 1 and 5 mg/L) was assessed. MS medium for apical segments cultivation was enriched with 0.1 mg/L indole-3-acetic acid (IAA) or combination of IAA and 0.1 mg/L N6-benzylaminopurin (BA). The production of gaseous hormone ethylene was analysed after 1, 2, 3, 7, 14 and 21 days of cultivation by GC-FID. In 21-day-old plants the level of cytokinins was analysed using the ELISA method and the level of ABA was analysed by RIA method. The content of endogenous phytohormones (ethylene, cytokinins (DHZR, iP, BA, BAR, mT, mTR) and ABA) significantly increased with increasing FLT concentration (1 and 5 mg/L) in the environment. This fact indicates that it could be a precious tool for the study of toxic effects of other environmental contaminants.

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