Publication details

Produkce metalothionenům podobného proteinu u Pseudomonas putida CCM 4307 v přítomnosti těžkých kovů

Title in English Production of methallothionein-like protein of Pseudomonas putida CCM 4307 in presence of heavy metals

NĚMEC Miroslav HAMMEROVÁ Jindřiška

Year of publication 2008
Type Article in Proceedings
Conference Proteiny 2008
MU Faculty or unit

Faculty of Science

Field Microbiology, virology
Keywords metallothionein; heavy metals
Description The aim of this work was to analyze effect of Cu, Ni, Cd and Zn on the biosynthesis of metallothionein-like protein. Studied organism was exposed to solution of metal chlorides in two concentrations - 7,82 and 15,63 mg/l. Results showed significant changes in synthesis of metallothionein-like proteins in presence of heavy metals. The biggest amount of MT-like proteins was detected when the studiedd mcroorganism was cultivated in presence of cadmium. The extraction system, composed from Bug Buster Protein Extractin Reagent (Novagen) and Lysonase (Novagen), was used for the extraction of MT-like protein. Purity of isolated proteins was checked by SDS-PAGE.
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