Využití PCR při rozlišení živých a mrtvých bakteriálních buněk

• Title in English: Differentiation of live and dead bacterial cells by PCR
• Authors: TRACHTOVÁ Štěpánka; ŠPANOVÁ Alena; RITTICH Bohuslav
• Year of publication: 2008
• Type: Article in Proceedings
• Conference: XII. Setkání biochemiků a molekulárních biologů
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: PCR; EMA; live and dead cells
• Description: Lactobacillus paracasei ssp.paracasei CCDM211/06 were used for confirmation of the method. Different methods were used for preparation of dead cells (control). The time of incubation of cell suspension with EMA as the time of irradiation of the sample with halogen lamp were tested. PCR was carried out using genus specific primers (Dubernet a spol., 2002).

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