Publication details

CHARACTERISATION OF A NEW LECTIN BCLC FROM THE HUMAN OPPORTUNISTIC PATHOGEN BURKHOLDERIA CENOCEPACIA

Authors

ŠULÁK Ondřej DELIA Monia KOSTLÁNOVÁ Nikola IMBERTY Anne WIMMEROVÁ Michaela

Year of publication 2008
Type Article in Proceedings
Conference XII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Biochemistry
Keywords Lectin; Burkholderia cenocepacia; SPR; ITC
Description Four genes coding proteins homologous to lectin PA-IIL from Pseudomonas aeruginasa have been found in the genome of B. cenocepacia. One of them, named BclC, is a 28 kDa protein which is able to recognize D-mannosylated carbohydrates. This protein also exhibits partial sequence homology with the protein CV-IIL from the Chromobacterium violaceum. BclC has been cloned and prepared in recombinant form. Sequence analysis showed two distinct domains in the protein, N-terminal part that does not have any sequence homolog in genomic and protein databases, and C-terminal part that codes for lectin domain. Both domains were also cloned separately to simplify further structure-function characterization. Detailed functional studies using surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) allowed to define binding properties (afinity, kinetics) and thermodynamic parameters.
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