Publication details

Identification of antifungal proteins in the barley and malt caryopsis by mass spectrometry

Authors

HÉGROVÁ Blanka MACUCHOVÁ Simona PREISLER Jan HAVEL Josef

Year of publication 2008
Type Article in Proceedings
Conference Vitamins 2008 Nutrition and Diagnostics
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords low molecular weight proteins; barley; malt; SDS-PAGE; enzymatic digestion; MALDI-TOF mass spectrometry
Description Recently, great attention is paid to determination of various metabolites after pathogen-caryopsis interaction. Some of antifungal proteins, e.g. thionins, might be of effect on barley quality and its subsequent food products - gushing or premature yeast flocculation. The changes proceeding during pathogen-caryopsis interaction were monitored in 20 samples of barley and its corresponding malt by the combination of Sodium Dodecyl Sulphate - Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix-Assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF MS).
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