MALDI MS profiling of bacteria

• Authors: ŠEDO Ondrej; VORÁČ Aleš; TESHIM Andrea; NEMEC Alexandr; TVRZOVÁ Ludmila; SEDLÁČEK Ivo; ZDRÁHAL Zbyněk
• Year of publication: 2009
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: MALDI MS of intact bacterial cells or their lysates appears to be a highly promising tool for bacterial identification. During the past decade, numerous laboratories demonstrated the ability of this technique to rapidly resolve bacterial samples, concerning mostly a model set of organisms characterised previously or in parallel by other taxonomical approaches. Almost every laboratory developed its own sample preparation protocol working in a satisfactory way on a particular system of interest. Many factors were found to influence the signal quality, sensitivity, reproducibility and other method characteristics resulting in notable changes in MALDI MS profiling discriminatory power.

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