Publication details

c-Myb expression at different stages of molar tooth germ

Authors

MATALOVÁ Eva RADLANSKI Ron MÍŠEK Ivan ŠMARDA Jan

Year of publication 2009
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description The aim of this work was to show c-myb expression profile in temporo-spatial model of mouse molar tooth germ development in the context of related tissues. Expression of c-myb (Abcam polyclonal antibody) was followed together with proliferation analysis (PCNAproliferating cell nuclear antigen, DAKO monoclonal antibody) and apoptosis detection (TUNEL test, terminal deoxynucleotidyl transferase mediated dUTP nick end labelling, Chemicon). Secondary biotinylated antibody was labelled by streptavidin-POD to visualize positive cells by addition of DAB substrate. c-myb, PCNA and TUNEL were evaluated in the epithelial and mesenchymal parts of the tooth germ and in the surrounding interdental bone.
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