Publication details

A combined AFLP-multiplex PCR assay for molecular typing of Escherichia coli strains using variable bacterial interspersed mosaic elements

Authors

HORVÁTH Radek DENDIS Miloš SCHLEGELOVA J. RŮŽIČKA Filip BENEDÍK J.

Year of publication 2004
Type Article in Periodical
Magazine / Source Epidemiology and Infection
MU Faculty or unit

Faculty of Medicine

Citation
Field Microbiology, virology
Keywords AFLP-multiplex PCR; molecular typing; Escherichia coli
Description The original method for molecular typing of E. coli strains was developed using the polymorphism in chromosomal sequences of bacterial interspersed mosaic elements (BIMEs) detected by Multiplex PCR and analysed by AFLP assay. The applicability of the method in the epidemiology of E. coli was tested on a group of 524 strains of human and veterinary origin. In the studied group 18 different genotypes were detected. Significant differences were found in the frequencies of the genotypes among various groups of strains, suggesting the method could be a promising tool in the epidemiology of E. coli.

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