Publication details

Fluoranthene influences endogenous abscisic acid level and primary photosynthetic processes in pea (Pisum sativum L.) plants in vitro

Authors

VÁŇOVÁ Lucie KUMMEROVÁ Marie KLEMŠ Marek ZEZULKA Štěpán

Year of publication 2009
Type Article in Periodical
Magazine / Source Plant Growth Regulation
MU Faculty or unit

Faculty of Science

Citation
Field Physiology
Keywords Abscisic acid; Chlorophyll fluorescence imaging; Fluoranthene; Indole acetic acid; Pea; Pisum sativum L.
Description The influence of increasing concentrations (0.1, 1.0 and 5.0 mg l-1) of fluoranthene (FLT) on growth, endogenous abscisic acid (ABA) level and primary photosynthetic processes in 21-day-old pea plants (Pisum sativum L.) in vitro was investigated. Murashige and Skoog (MS) medium, with or without FLT, was enriched with indole-3-acetic acid (IAA; 0.1 mg l-1) or a combination of IAA(0.1 mg l-1) plus N6-benzyladenine (BA; 0.1 mg l-1). The level of endogenous ABA significantly increased with increasing FLT concentrations in the presence of both IAA and IAA plus BA. An increased level of endogenous ABA was observed in plants treated with IAA alone. The growth of shoot, callus and the content of photosynthetic pigments (chlorophyll a and b, carotenoids), in both IAA- and IAA plus BA-treated plants, were significantly stimulated by FLT at its lowest concentration (0.1 mg l-1) assayed in this study. However, FLT at higher concentrations (1.0 and 5.0 mg l-1) significantly inhibited all these parameters. Chlorophyll fluorescence imaging showed that FLT only at the highest concentration (5.0 mg l-1) in the presence of IAA (0.1 mg l-1) significantly increased F0, but decreased FV/FM and PhiII.