Publication details
Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | ANALYTICAL AND BIOANALYTICAL CHEMISTRY |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | Nitric oxide; Macrophages; RAW 264.7; Nitric oxide sensor; Nitrites; iNOS expression |
| Description | In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment. |