Publication details

Fungicides and insecticides effects on photosynthesis and photoprotective mechanisms in A. thaliana under high light and high temperature stress



Year of publication 2009
Type Conference abstract
MU Faculty or unit

Faculty of Science

Description In cultivations of the most common experimental plant A. thaliana, chemical control is unevoidable. We invesigated co-effects of fungicides/insecticides and high light/heat treatments. Wild type A. thaliana (Columbia) and LHC-defficient mutants (Lhcb 2-1) were used and treated each 30 d by the following chemicals: (1) Previcur 607SL, (2) Zolone 35EC, (3) a mixture of 0,25% Previcur and 0,2% Zolone, and (4) untreated control. Additionally, the plants were exposed to 2 short-term treatmets: (a) 1000 micromol m-2 s-1 of PAR (high light = HL) for 60 min and (b) combination of HL and 30 oC. Treatment effects on photosynthesis were monitored by a PAM-2000 fluorometer. Before and after the treatments, pigments were extracted from leaf tissue and HPLC determined: contents of chla, chlb, viola-, anthera-, zeaxanthin, lutein, beta-carotene were evaluated by a HPLC (Waters). Results showed that Previcur plus Zolone caused oxidative stress in chloroplasts even in control plants indicaded by increased values of zeaxanthin and DEPS. In Lhcb mutants, Fv/Fm decreased after HL and temperature treatments which was attributed to negative short-term effects in photosystem II. Supported by FRVS 1546/2008.
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