PREPARATION AND MODIFICATION OF QUANTUM DOTS AS SELECTIVE PROBES IN BIOANALYSIS
|Year of publication
|Article in Proceedings
|ChemZi 5/9 2009, 61. Zjazd chemikov, Vysoké Tatry, Slovensko
|MU Faculty or unit
|Quantum dots ;photoluminescent probes;immunolabeling
|Quantum dots (QD) show a great potential as photoluminescent probes at cellular level, including in vivo immunolabeling or cellular tracking. The main advantages of quantum dots, when compared with the conventional organic fluorescent dyes, are practically no photobleaching, wide excitation and narrow emission spectra and size dependent emission wavelength. Typical materials for the preparation of quantum dots are elements of II - VI or III - V group. We have prepared CdTe water soluble quantum dots of sizes 2 - 4,5 nm by the chemical reaction between CdCl2 and NaHTe at the presence of 3-mercaptopropionic acid. Thus, the photoluminescence emission maxima reach the values 520 - 700 nm. The emission spectra are narrow with the bandwidth of about 58 nm at the half height of its intensity, while the excitation band lies in the range from 300 to 550 nm. There is a lot of simple conjugation techniques for linking of QD with biomolecules to use them as selective probes in immunolabeling.