Publication details
Application potential of electrophoretically mediated microanalysis
| Authors | |
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| Year of publication | 2010 |
| MU Faculty or unit | |
| Citation | |
| Description | Today, electrophoretically mediated microanalysis (EMMA) attracts the attention for its prospective applications. EMMA method belongs to techniques of capillary electrophoresis (CE) and thus for the first time CE does not serve as a separation tool only. Thanks to the EMMA methodology it represents the technique which combines reaction and separation in the confines of capillary column. The first work, in which this in-line arrangement was used, was published by Bao and Regnier [1]. Subsequently the EMMA method has been utilized for a range of biochemical systems comprising assays of enzyme activity and kinetics and determination of substrates, inhibitors, Michaelis constants, and inhibition constants. Later on the EMMA methodology was introduced into non-enzymatic systems as derivatization reactions and examination of chemical reactions in microscale. Together with advantages of CE techniques EMMA method shows huge application potential in different fields of research. The main assets of this complex method are full integration of all reaction steps, minimal consumption of reagents and high degree of automation. The examples of enzymatic reactions from the field of proteomics and drug metabolism studies were presented. |
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