Publication details
Lectin from Chromobacterium violaceum - role of water molecule in the carbohydrate binding site
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | CV-IIL is a lectin from human opprotunistic pathogen microorganism Chromobacterium violaceum. Crystal structure of this lectin demonstrates, that there is one water molecule in the carbohydrate binding site, which plays a special role by bridging sugar with backbone nitrogen atom and also with side chain of the amino acid threonine in position 97. Substitution of this amino acid should lead to changes of thermodynamical parametres (an enthalpic and entropic contribution to the free energy of the interaction lectin-sugar) that could be determine by an isothermal titration microcalorimetry method. |
| Related projects: |