Oligonucleotide array-based comparative genomic hybridization analyses of gain 1q21 in multiple myeloma

• Authors: ZAORALOVÁ Romana; NĚMEC Pavel; GREŠLIKOVÁ Henrieta; SMETANA Jan; KOVÁŘOVÁ Lucie; HÁJEK Roman; KUGLÍK Petr
• Year of publication: 2010
• Type: Conference abstract
• MU Faculty or unit: Faculty of Medicine
• Description: Using oligonucleotide array-CGH we analyzed 41 MM samples with gain of 1q21 (CKS1B gene) detected previously by FISH. As input material for array-CGH we used DNA from CD138+ cells separated from bone marrow using magnetic or fluorescence activated cell separation. Taking together 76% (13 of 41) cases had gained not only 1q21 locus but whole 1q arm. Moreover, another 17% (7 of 41) had at least 100 MB gain anywhere at the 1q arm. It is obvious, that not only CKS1B gene, but other genes are affected as well, for example IL-6R or BCL6. Many of them are possibly negative prognostic factors in MM. According to the FISH data (in about 95% cases we detect max. two extra copies of CKS1B), we did not find any high-level amplification of 1q21. In all cases, 1q aberrations were associated with additional copy number alterations, including monosomy of chromosome 13 or hyperdiploidy.

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