Publication details

Rapid detection and identification of mucormycetes from culture and tissue samples by use of high-resolution melt analysis

Authors

HRNČÍŘOVÁ Kristýna LENGEROVÁ Martina KOCMANOVÁ Iva RÁČIL Zdeněk VOLFOVÁ Pavlína PALOUŠOVÁ Dita MOULIS Mojmír WEINBERGEROVÁ Barbora WINTEROVÁ Jana TOŠKOVÁ Martina POSPÍŠILOVÁ Šárka MAYER Jiří

Year of publication 2010
Type Article in Periodical
Magazine / Source Journal of Clinical Microbiology
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1128/JCM.01109-10
Field Oncology and hematology
Keywords Mucormycetes; rapid detection; simple detection; real-time PCR
Description We present a method for rapid and simple detection of clinically relevant mucormycetes of the Mucorales order in cultures and clinical samples. This seminested real-time PCR uses mucormycete-specific primers and is followed by species identification using high-resolution melt (HRM) analysis. The method is highly suitable for routine clinical diagnostics.

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