Publication details
Isolation of Phosphoproteom and its Application in Study of the Effect of Cytokinin on Plants
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| MU Faculty or unit | |
| Citation | |
| Web | http://web2.mendelu.cz/af_291_mendelnet/mendelnet08agro/files/articles/biolrost_cerny.pdf |
| Description | Phosphorylation is one of the most important posttranslational modifications in proteins. It is also a major issue in modern proteomics. Its application in plant research is rather scarce. We present here development of optimized protocol for phophoprotein enrichment kit (Quiagen) for extraction of plant phosphoproteom. This protocol allows reusing of affinity column designated for single use only up to 3 times with phosphoproteom quality unaffected. We also provide example of application of this improved protocol in research of cytokinin activation pathway in plants. |