Publication details
Ultrastructure of the cell wall of Schizosaccharomyces pombe following treatment with various glucanases
| Authors | |
|---|---|
| Year of publication | 1995 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Structural Biology |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=CitationReport&qid=13&SID=Z16b286IgGDC9gP7KFB&page=1&doc=4 |
| Field | Morphological specializations and cytology |
| Keywords | Ultrastructure; cell wall; yeast; Schizosaccharomyces pombe; various glucanases |
| Description | The ultrastructure of isolated cell walls of Schizosaccharomyces pombe was studied by electron microscopy after treatment with the following purified enzymes: endo-beta-1,3-glucanase, endo-beta-1,6-glucanase, and endo-alpha-1,3-glucanase produced by Bacillus circulans; exo-beta-1,3-glucanase and endo-beta-1,3-glucanase produced by Schizosaccharomyces japonicus var. versatilis. The exo-beta-1,3-glucanase had no detectable effect on the walls, but amorphous wall material was removed by action of the endo-beta-1,3- and endo-beta-1,6-glucanases of B. circulans to reveal a wall component consisitng of densely interwoven microfibrils. The fibrils were hydrolysed by treatment with the Schiz. japonicus endo-beta-1,3-glucanase-suggesting that they were composed of beta-1,3-linked glucan and alpha-1,3-linked glucan. The presence of a fibrillar component in untreated walls was evident after negative staining. |