Publication details

Action of ribonuclease on growth, synthesis of cell wall and ultrastructure in yeast protoplasts and cells.

Authors

KOPECKÁ Marie SVOBODA Augustin NEČAS Oldřich

Year of publication 1969
Type Article in Periodical
Magazine / Source ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY AND SEROLOGY
MU Faculty or unit

Faculty of Medicine

Citation
Web http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=T241EKEk3JJl1LjneHl&page=6&doc=52
Field Morphological specializations and cytology
Keywords yeast protoplasts; ribonuclease effect; wall synthesis
Description Action of ribonuclease on growth, synthesis of cell wall and ultrastructure in yeast protoplasts and cells was studied by light and electron microscopy.It was possible to find concentration of ribonuclease at which protoplasts are viable, but do not grow and vacuolize. These protoplasts are not able to synthesize the glucan fibrila of the wall, probably due to damage of plasma membrane by ribonuclease - plasma membrane probably takes part in the synthesis of fibrils. Ultrastructure of plasma membrane was affected by ribonuclease, as shown by freeze-fracture and transmission electron microscopy.

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