Publication details

Pentapeptide-modified poly(N,N-diethylacrylamide) hydrogel scaffolds for tissue engineering

Authors

HORÁK Daniel MATULKA Kamil HLÍDKOVÁ Helena LAPČÍKOVÁ Monika BENEŠ Milan J. JAROŠ Josef HAMPL Aleš DVOŘÁK Petr

Year of publication 2011
Type Article in Periodical
Magazine / Source Journal of Biomedical Materials Reseach Part B: Applied Biomaterials
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1002/jbm.b.31832
Field Genetics and molecular biology
Keywords poly(NN-diethylacrylamide); porosity; YIGSR; peptide; embryonic stem cells; hydrogel
Description Poly(N,N-diethylacrylamide) (PDEAAm) hydrogel scaffolds were prepared by radical copolymerization of N,Ndiethylacrylamide (DEAAm), N,N0-methylenebisacrylamide and methacrylic acid in the presence of (NH4)2SO4 or NaCl. The hydrogels were characterized by low-vacuum scanning electron microscopy in the water-swollen state, water and cyclohexane regain, and by mercury porosimetry. The pentapeptide, YIGSR-NH2, was immobilized on the hydrogel. Human embryonic stem cells (hESCs) were cultured with the hydrogels to test their biocompatibility. The results suggest that the PDEAAm hydrogel scaffolds are nontoxic and support hESC attachment and proliferation, and that interconnected pores of the scaffolds are important for hESC cultivation. Immobilization of YIGSR-NH2 pentapeptide on the PDEAAm surface improved both adhesion and growth of hESCs compared with the unmodified hydrogel. The YIGSRNH2- modified PDEAAm hydrogels may be a useful tool for tissue-engineering purposes.
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