Publication details

Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities

Authors

ŠEBESTA Marek BURKOVICS Peter HARACSKA Lajos KREJČÍ Lumír

Year of publication 2011
Type Article in Periodical
Magazine / Source DNA Repair
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1016/j.dnarep.2011.03.003
Field Genetics and molecular biology
Keywords DNA repair; Recombination; DNA synthesis; Replication; Mph1 Srs2
Description The error-free repair of double-strand DNA breaks by homologous recombination (HR) ensures genomic stability using undamaged homologous sequence to copy genetic information. While some of the aspects of the initial steps of HR are understood, the molecular mechanisms underlying events downstream of the D-loop formation remain unclear. Therefore, we have reconstituted D-loop-based in vitro recombinationassociated DNA repair synthesis assay and tested the efficacy of polymerases Pol and Pol to extend invaded primer, and the ability of three helicases (Mph1, Srs2 and Sgs1) to displace this extended primer. Both Pol and Pol extended up to 50% of the D-loop substrate, but differed in product length and dependency on proliferating cell nuclear antigen (PCNA). Mph1, but not Srs2 or Sgs1, displaced the extended primer very efficiently, supporting putative role ofMph1in promoting the synthesis-dependent strand-annealing pathway. The experimental system described here can be employed to increase our understanding of HR events following D-loop formation, as well as the regulatory mechanisms involved.
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